Synthesis of Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Salbutamol from Urine Samples
ALI MOHAMMADI* , TAHER ALIZADEH! , RASSOUL DINARVAND , MOHAMMAD REZA GANJALI! and RODERICK B. WALKERº

Department of Drug and Food Control, School of Pharmacy
Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran 14174, Iran
Fax: (98)(21)6461178; Tel: (98)(21)6959090; E-mail: alimohammadi@tums.ac.ir

Abstract

A highly selective methacrylic based molecularly imprinted polymer (MIP) was synthesized and applied for the separation and the pre-concentration of salbutamol in urine samples. Spectrophotometric determination of salbutamol was achieved using 2,6-dichloroquinone chlorimide as colorimetric reagent. The detection limit of the method was ca. 13 ng mL-1 in urine after pre-concentration of the samples by MIP-SPE and analysis with an optimized and sensitive spectrophotometric method. The linear dynamic range for salbutamol determination in urine was 0.04-0.75 μg mL-1. The recovery for the affinity based solid-phase extraction (SPE) with the MIP was more than 96 %.

Keywords

d u Salbutamol, 2,6-Dichloroquinone chlorimide, Molecularly imprinted polymer, Solid-phase extraction.
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  • Asian J. Chem. /
  •  2009 /
  •  21(4) /
  •  pp 2875-2880