Study on Identification and Separation of the Antifungal Antibiotic from its Fermentation Broth of Streptomyces hygroscopicus BOS-013 Strain

Wang Yong1,*, Zhang Wei1, Zhang Wen-Ge2, Wang Ning-Ning1, Lu Dan1, Zhou Lin1 and Yan Cong-Yang1

1University of Science and Technology, Anshan 114044, Liaoning Province, P.R. China

2Anshan Normal University Affiliated Medical School, Anshan 114001, P.R. China

*Corresponding author: Fax: +86 412 5929480; Tel: +86 412 5929030; E-mail: askdzw@163.com

Abstract

BOS-013 actinomycete strain was obtained by separating difference soil of areas from Chang Bai Mountain. The antifungal antibiotic produced by Streptomyces hygroscopicus BOS-013 was purified by means of macro-porous adsorbent resin and thin-layer chromatography. The separation and purification of the antifungal antibiotic from its fermentation broth of Streptomyces hygroscopicus BOS-013 strain by simulated moving bed chromatography and the crystal of the antibiotic with high purity was obtained. In this paper, the methods of purification by adsorbing of microporous adsorbent resin and detection by high performance liquid chromatography with mass spectrum (HPLC-MS) were established. The study lays a good foundation for its physical-chemical properties. Meanwhile,we identified its structure by spectral analyses. Quasi-molecular ion peak [M + H]+ was 547 given by positive ion mode-electrospray ionization-mass spectrometry and pushed-out its molecular weight was 546. Its molecular formula was C30H43O9. We did not find this compound, so it was infered as a new compound temporarily after Scifinder search.

Keywords

Streptomyces, Screen, Antibiotic, Isolation and purification, Identification.

   View Article PDF File Under a Creative Commons License
 
  • Asian J. Chem. /
  •  2012 /
  •  24(9) /
  •  pp 3821-3824