On-Line Solid-Phase Extraction of Fluoroquinolone Residues from Milk with Enrofloxacin-Imprinted Monolithic Column

Yun-Kai Lv*, Cui-Ling Jia, Xiao-Hu Wang and Li-Wei Zhang

College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, P.R. China

*Corresponding author: Fax:+ 86 312 5079628; Tel: +86 312 5079359; E-mail: lvyunkai@hbu.edu.com

Abstract

On-line solid-phase extraction method was developed by enrofloxacin-imprinted monolithic column (monolithic MIP) coupling with reversed-phase high-performance liquid chromatography for preconcentration and separation of fluoroquinolone residues in milk. The monolithic MIP was prepared by in situ polymerization in a stainless-steel chromatographic column. An enrichment factor of 6.70 along with a good sample clean-up effect was obtained. High recoveries for three fluoroquinolones such as enrofloxacin, ciprofloxacin and norfloxacin were achieved, which was in the range of 82.0-94.0 % with precision lower than 5.6 %. The limit of detection and limit of quantitation of the proposed method were in a range of 4.31-7.62 μg/kg and 14.37- 25.40 μg/kg, respectively. The proposed method was successfully applied to on-line preconcentration, separation and determination of the fluoroquinolones in the milk sample.

Keywords

Molecularly imprinted monolithic column, On-line, Solid-phase extraction, Enrofloxacin, Milk.

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  • Asian J. Chem. /
  •  2012 /
  •  24(9) /
  •  pp 3913-3916