Extractive Bioconversion of L-Phenylalanine to 2-Phenylethanol Using Polypropylene Glycol 1500

Dong-Liang Hua1, Xiao-Hui Liang1, Cheng-Chuan Che2, Xiao-Dong Zhang1,*, Jie Zhang1, Yan Li1 and Ping Xu3

1Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, P.R. China

2School of Life Sciences, Qufu Normal University, Qufu 273165, P.R. China

3School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P.R. China

*Corresponding author: Fax: +86 531 82961954; Tel: +86 531 85599031; E-mail: hua016@foxmail.com


Product cytotoxicity was a bottleneck in biotechnological production of 2-phenylethanol from L-phenylalanine. In this study, polypropylene glycol 1500 (PPG 1500) was firstly used as an extractant in the extractive bioconversion of L-phenylalanine by strain Saccharomyces cerevisiae P-3. About 4.8 g L-1 2-phenylethanol was obtained from 7 g L-1 L-phenylalanine in flask experiment within 36 h, with a high molar yield of 92.7 %. Compared to conventional bioconversion without addition of extractant, the yield was improved by 26.3 %. In a fed-batch extractive bioconversion, the highest concentration of 2-phenylethanol in the organic phase exceeded 22 g L-1 and the total concentration in both aqueous and organic phases reached 7.5 g L-1. The results showed that the process with polypropylene glycol 1500 as an extractant might have the potential in bioproduction of 2-phenylethanol.


2-Phenylethanol, L-phenylalanine, Product cytotoxicity, Polypropylene glycol 1500, Extractive bioconversion.

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