Determination of Cefoperazone Sodium in Pharmaceutical Formulations by Fe3+-Phenanthroline Spectrophotometry

Ou Sha1,2,*, Yin Liao1, Yun Zhou1, Si Liang Zhang1 and Weixing Ma1

1School of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P.R. China

2Analysis and Test Center of Jiangsu Marine Resources Development Research Institute, Lianyungang 222001, P.R. China

*Corresponding author: Tel: +86 518 85895063; E-mail:


A new method for determination cefoperazone by spectrophotometry was developed based on Fe3+ as the oxidizer of cefoperazone and phenanthroline as the colouring reagent of Fe2+, which was produced from Fe3+. Under the optimum conditions, the relationship between the absorbance and the concentration of cefoperazone was linear in the range of 0.50-12.50 mg/L, the regression equation was A = 0.1179 c (c: mg/L) +0.0003, the relative coefficient was 0.9985. The proposed methods have been applied to the determination of cefoperazone content in pharmaceutical formulations with satisfactory results.


Spectrophotometry, Cefoperazone, Phenanthroline.

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