Selective Adsorption of Berberine Hydrochloride Using Molecularly Imprinted Polymers with Modified Rosin as Cross-linker

Hao Li1,2, Fuhou Lei1,2,*, Pengfei Li1,2, Wengui Duan3, Juying Zhou1,2 and Xuecai Tan1,2

1School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, P.R. China

2Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, P.R. China

3School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, P.R. China

*Corresponding author: Fax: +86 771 3261718; Tel: +86 771 3262198; E-mail: leifuhou@gmail.com

Abstract

Molecularly imprinted polymers containing phenanthrene skeleton were prepared via suspension polymerization with ethylene glycol maleic rosinate acrylate (EGMRA) as a cross-linker, berberine hydrochloride as the template and methacrylic acid as the functional monomer. The binding characteristics of the molecularly imprinted polymers and their templates were evaluated via equilibrium binding, whereas those of berberine hydrochloride on molecularly imprinted polymers were evaluated via Scatchard analysis. The adsorption energy was measured via microcalorimetry, whereas the selective recognition of molecularly imprinted polymers was investigated through high-performance liquid chromatography. Scatchard plot analysis reveals the formation of two types of binding sites in the molecularly imprinted polymers, with their respective equilibrium dissociation constants at Kd1 = 0.0204 and Kd2 = 0.0507 mmol·L-1. The heat flow curve exhibits an endothermic peak and two relatively weak exothermic peaks for the adsorption. Selective adsorption results demonstrate the high affinity and berberine hydrochloride selectivity of the molecularly imprinted polymers.

Keywords

Berberine hydrochloride, Molecularly imprinted polymers, Selective adsorption, Microcalorimetry, Modified rosin.

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