Determination of DL-Methionine with Electro-chemiluminescence Molecularly Imprinting Method

Zhen Du1,2 and Chenling Fan3,*

1Department of Pharmacy, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Road, Heping Dist. 110001, Shenyang Liaoning Province, P.R. China

2School of Pharmacy, China Medical University, No. 92 Beier Road, Heping Dist. 110001, Shenyang Liaoning Province, P.R. China

3Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, Shenyang 110001, P.R. China

*Corresponding author: Tel: +86 13700004043; E-mail: zhong-shan2000@hotmail.com

Abstract

In order to establish a novel EC-MIP method in the determination of DL-methionine in human urine, a carbon electrode of [Ru(bpy)3]2+ was modified with the molecular imprinting polymers electro-deposited with the sol-gel method. The abilities of the electrochemical and electro-chemiluminescence characteristics of the above electrode were then investigated with the quantitative determination of dL-methionine in human urine. The method exhibited the high sensitivity and excellent selectivity towards DL-methionine. With the optimized conditions, the linear range of dL-methionine was from 1 × 10-11 mol/L to 1 × 10-8 mol/L, with the LOD as 2.6 × 10-12 mol/L. This method could be successfully applied to the determination of dL-methionine in human urine samples for the precaution of certain diseases.

Keywords

Electro-chemiluminescence, Molecularly imprinting method, DL-Methionine.

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