Determination of Prilocaine HCl in Pharmaceutical Preparations by GC-MS Method

Alptug Atila and Yucel Kadioglu*

Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240 Erzurum, Turkey

*Corresponding author: Fax: +90 442 2360962; Tel: +90 442 2315212; E-mail: yucelkadi@yahoo.com

Abstract

The novel analytical method using gas chromatography with mass spectrometry detection (GC-MS) method for determination of prilocaine HCl in pharmaceutical preparations were developed and validated. The using lidocaine HCl as the internal standard (IS) was carried out on HP-5MS capillary column. The calibration curves were linear (R = 0.999) in concentration range of 40-1000 ng/mL of prilocaine HCl. The LOD and LOQ values were 15 and 31 ng/mL, respectively. The within- and between-day RSD and RE % values of proposed method were found to be £ 3.4 and £ 3.5 %, respectively. o-Toluidine was formed in stored on auto sampler during 72 h was determined by proposed method. The developed method can be directly and easily applied for determination of prilocaine HCl without derivatized in pharmaceutical preparations using internal standard methodology.

Keywords

GC-MS method, Prilocaine HCl, Pharmaceutical preparations, Validation.

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