Molecular Imprinted Polysiloxanes for Selective Removal of Leucomalachite Green

Muhammad Naveed Khan1, Muhammad Irshad1, Adnan Mujahid1,*, Tajamal Hussain1, Muhammad Hamid Raza1, Mirza Nadeem Ahmad2, Arslan Mujahid3 and Muhammd Umar Farooq4

1Institute of Chemistry, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan

2Institute of Chemistry, Government College University, Faisalabad-38030, Pakistan

3College of Earth and Environmental Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan

4Institute of Environmental Engineering and Research, University of Engineering and Technology, Lahore-54890, Pakistan

*Corresponding author: Fax: +92 42 99230998; Tel: +92 42 99230463 (Ext. 844); E-mail:


Leucomalachite green sensitive molecular imprinted polysiloxanes were synthesized by sol-gel polymerization. The imprinting of leucomalachite green in polymer matrix was investigated by FTIR studies which indicate the presence of leucomalachite green characteristic peaks. Washing imprinted polysiloxanes with deionized water leads to the removal of leucomalachite green thus, developing adapted cavities for reversible re-inclusion of analyte molecules. The recognition properties of imprinted polysiloxanes were evaluated by their rebinding affinity towards leucomalachite green. In rebinding studies, imprinted polysiloxanes showed significant binding affinity for leucomalachite green i.e. tested in the range of 0.04 to 0.1 mmol whereas the non-imprinted polysiloxanes exhibited much lower binding. Imprinted polysiloxanes were highly selective for leucomalachite green recognition as they showed five-fold higher binding response when compared with structurally related methyl violet dye. Finally, their reusability was evaluated which indicated that the rebinding efficiency of regenerated polysiloxanes was more than 80 % comparing to freshly prepared polymer suggesting them highly cost effective and could be used as potential recognition matrix for leucomalachite green removal from environmental samples.


Leucomalachite green, Molecular imprinting, Polysiloxanes, Methyl violet.

Reference (17)

1.      D.R. Kryscio and N.A. Peppas, Acta Biomater., 8, 461 (2012); doi:10.1016/j.actbio.2011.11.005.

2.      F.L. Dickert and O. Hayden, Anal. Chem., 74, 1302 (2002); doi:10.1021/ac010642k.

3.      L.D. Bolisay, J.N. Culver and P. Kofinas, Biomaterials, 27, 4165 (2006); doi:10.1016/j.biomaterials.2006.03.018.

4.      U. Latif, A. Mujahid, A. Afzal, R. Sikorski, P.A. Lieberzeit and F.L. Dickert, Anal. Bioanal. Chem., 400, 2507 (2011); doi:10.1007/s00216-011-4927-1.

5.      M. Irshad, N. Iqbal, A. Mujahid, A. Afzal, T. Hussain, A. Sharif, E. Ahmad and M. Athar, Nanomaterials, 3, 615 (2013); doi:10.3390/nano3040615.

6.      C. He, Y. Long, J. Pan, K. Li and F. Liu, J. Biochem. Biophys. Methods, 70, 133 (2007); doi:10.1016/j.jbbm.2006.07.005.

7.      D. Cunliffe, A. Kirby and C. Alexander, Adv. Drug Deliv. Rev., 57, 1836 (2005); doi:10.1016/j.addr.2005.07.015.

8.      V. Pichon and F. Chapuis-Hugon, Anal. Chim. Acta, 622, 48 (2008); doi:10.1016/j.aca.2008.05.057.

9.      S.A. Piletsky, N.W. Turner and P. Laitenberger, Med. Eng. Phys., 28, 971 (2006); doi:10.1016/j.medengphy.2006.05.004.

10.  C.-J. Cha, D.R. Doerge and C.E. Cerniglia, Appl. Environ. Microbiol., 67, 4358 (2001); doi:10.1128/AEM.67.9.4358-4360.2001.

11.  K. Mitrowska, A. Posyniak and J. Zmudzki, J. Chromatogr. A, 1089, 187 (2005); doi:10.1016/j.chroma.2005.07.004.

12.  A. Swarbrick, E.J. Murby and P. Hume, J. Liq. Chromatogr., 20, 2269 (1997); doi:10.1080/10826079708006562.

13.  P. Scherpenisse and A.A. Bergwerff, Anal. Chim. Acta, 529, 173 (2005); doi:10.1016/j.aca.2004.08.009.

14.  G. Dowling, P.P.J. Mulder, C. Duffy, L. Regan and M.R. Smyth, Anal. Chim. Acta, 586, 411 (2007); doi:10.1016/j.aca.2006.08.045.

15.  P. Ngamukot, T. Charoenraks, O. Chailapakul, S. Motomizu and S. Chuanuwatanakul, Anal. Sci., 22, 111 (2006); doi:10.2116/analsci.22.111.

16.  U. Divrikli, A.A. Kartal, M. Soylak and L. Elci, J. Hazard. Mater., 145, 459 (2007); doi:10.1016/j.jhazmat.2006.11.040.

17.  A. Mittal, J. Hazard. Mater., 133, 196 (2006); doi:10.1016/j.jhazmat.2005.10.017.

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