Chemical Composition, Antioxidant and Antimicrobial Activity of the Essential Oil of Phellodendron amurense (Rupr.) from China

Y. Han1,2, L. Xu1,*, Q. Wang1, Y. Huang1 and W. Meng3

1College of Agricultural Resource and Environment, Heilongjiang University, Harbin, P.R. China

2College of Agronomy, Northeast Agricultural University, Harbin, P.R. China

3College of Life Science, Northeast Forestry University, Harbin, P.R. China

*Corresponding author: Fax: +86 451 86609487; Tel: +86 13694518965; E-mail:


The essential oil of Phellodendron amurense has been isolated from fruits collected in their native habitat China. Hydrodistillation of P. amurense afforded a pale yellow oil in 1.4 % (w/w) yield. The chemical composition of this essential oil was analyzed by GC-MS. Twenty-one compounds were identified, accounting for 97 % of the total oil. There were five most abundant constituents (greater than 5 %) were myrcene (51.7 %), 2-methyl-6-methyleneocta-3,7-dien-2-ol (7.4 %), 1,2-benzenedicarboxylic acid-bis(2-methylpropyl)-ester (7.2 %), 2-methyl-6-methyleneocta-1,7-dien-3-ol (7.1 %) and a-phellandrene (5.2 %).The essential oil of P. amurense showed antioxidant activity (IC50, 2.32 μg/mL) and broad spectrum fumigant and contact antimicrobial activity against all microbial strains tested (MIC or IC50, 0.12-1.36 μg/mL). These findings suggest that the essential oil may be used as an antioxidant or antimicrobial agent.


Phellodendron amurense, Essential oil, Antimicrobial activity, Rutaceae.

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