Analysis of 5,8,11,14,17-Eicosapentaenoic Acid and 4,7,10,13,16,19-Docosahexaenoic Acid in the Viscera of Marine Organisms Using Gas Chromatography

Deyong Zhang1,*, Xiaolu Xu1, Xiu-Ying Shen2 and Huiying Xu1

1College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, P.R. China

2College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, P.R. China

*Corresponding author: Fax: +86 571 88297098; Tel: +86 571 88298514; E-mail:


A gas chromatography was established for analysis of 5,8,11,14,17-eicosapentaenoic acid and 4,7,10,13,16,19-docosahexaenoic acid in the viscera of marine organisms. The viscera of 6 kinds of non-fish marine organisms were then collected for oil extraction and detection of 4,7,10,13,16,19-docosahexaenoic acid and 5,8,11,14,17-eicosapentaenoic acid. The oil extraction ratio varied from 0.72 to 6.23 % (wt. %). Scylla serrata presented the highest oil yield, followed by Architeuthis dux and Panopea abrupta. The 5,8,11,14,17-eicosapentaenoic acid concentration (in methyl ester form) in the oil varied from 0.56 to 7.75 (mg/g). Scylla serrata presented the highest 5,8,11,14,17-eicosapentaenoic acid concentration (p < 0.05), followed by Architeuthis dux and Panopea abrupta. The 4,7,10,13,16,19-docosahexaenoic acid concentration(in methyl ester form) varied from 0.15 to 4.71 (mg/g). Architeuthis dux and Scylla serrata presented obviously higher 4,7,10,13,16,19-docosahexaenoic acid concentration than other species (p < 0.05).


Marine organisms, Viscera, EPA, DHA, Gas chromatography.

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