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Studies on in vitro Antioxidant and Free Radical Scavenging Activities of Ethanol Extract of Whole Plant Biophytum sensitivum (Linn.) DC

B.R. Abhirama1, R. Shanmuga Sundaram2,* and R. Sambath Kumar3

1Department of Pharmaceutical Chemistry, J.K.K. Nattraja College of Pharmacy, Komarapalayam-638 183, India

2Department of Pharmacology, J.K.K. Nattraja College of Pharmacy, Komarapalayam-638 183, India

3Department of Pharmaceutics, J.K.K. Nattraja College of Pharmacy, Komarapalayam-638 183, India

*Corresponding author: Fax: +91 4288 264346; E-mail: malshan34@gmail.com

Abstract

The whole plant ethanol extract of Biophytum sensitivum Linn. DC (EEBS) was evaluated for its total phenolic content, total flavonoid content, free radical scavenging activities and reducing power. Phytochemical analysis revealed the presence of carbohydrates, alkaloids, steroids, saponins, tannins, phenolic compounds and flavonoids. The total phenolic contents in the extract was estimated by Folin-Ciocalteu reagent and indicated that extract contained 53.55 mg of GA/g of extract. The total flavonoid contents in the ethanol extract of Biophytum sensitivum Linn. DC were found to be 153.08 mg of RU/g of extract. The IC50 value of DPPH radical, ABTS radical, hydroxyl radical and superoxide radical scavenging activities were found to be 46.34, 42.01, 94.42, 72.12 μg/mL respectively. The IC50 value is defined as the concentration that causes a decrease in the initial amount of free radical by 50 %. The IC50 value in FRAP assay was found to be 52.12 μg/mL. Ethanol extract of Biophytum sensitivum Linn. DC at 80 μg/mL and 100 μg/mL showed higher reducing activity than standard ascorbic acid. The result of the present study showed that ethanol extract of Biophytum sensitivum Linn. DC possessed significant antioxidant and free radical scavenging potential and this may be attributed to the presence of bioactive phyto-consitutents like amentoflavone, a bioflavonoid with trace amounts of cupressoflavone, luteolin, isoorientin and isovitexin.

Keywords

Biophytum sensitivum, in vitro Antioxidant, ROS, Phenolic compounds, DPPH, Flavonoids.

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