Enzymatic Synthesis of Epoxidized Fatty Hydrazides from Epoxidized Palm Oil Using Native Muchor meihei Lipase in n-Hexane Solvent

Z. Jamingan1,*, W.M.Z.W. Yunus1,2, O.K. Khim3 and N. Abdullah3

1Faculty of Defence Science and Technology, National Defence University of Malaysia, Sg Besi Camp, 57000 Kuala Lumpur, Malaysia

2Centre for Tropicalization, Universiti Pertahanan Nasional Malaysia, Kem Sungai Besi, 57000 Kuala Lumpur, Malaysia

3Centre for Defence Foundation Studies, Universiti Pertahanan Nasional Malaysia, Kem Sungai Besi, 57000 Kuala Lumpur, Malaysia

*Corresponding author: E-mail: zakiahjamingan@yahoo.com

Abstract

Epoxidized fatty hydrazides have been synthesized through an enzymatic catalytic synthesis reaction of epoxidized palm oil with hydrazine monohydrate by native Muchor meihei lipase enzyme in n-hexane solvent. Optimum reaction conditions were studied by observing the yield of epoxidized fatty hydrazides production using several parameters. The highest production of epoxidized fatty hydrazides was obtained when reaction temperature was 50 ºC with an addition of 5 % of enzyme to catalyze the reaction. This were accompanied using stirring speed of 165 rpm under 24 h of reaction. The epoxidized fatty hydrazides was further characterized using Fourier transform infrared spectroscopy, nuclear magnetic resonance spectrometry and CHN elemental analyzer.

Keywords

Enzymatic synthesis, Epoxidized palm oil, Fatty hydrazides.

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