Quantitation of Vitamin E in Pharmaceutical Formulations and Oil Samples Using Spectrophotometric Method

Ashwani Kumar* and Mamta Kamboj

Department of Chemistry, Kurukshetra University, Kurukshetra-136119, India

*Corresponding author: E-mail: akumarchem@kuk.ac.in

Abstract

A novel, rapid and facile procedure is described for the determination of vitamin E. It involves the reduction of Fe(III) to Fe(II) with vitamin E that results the formation of pink colored complex of reduced Fe(II) with dimethyl glyoxime in presence of pyridine in pH range between 6.5-7.2. The absorbance of the complex was measured at 512 nm. Beer′s law was obeyed in the range 2.0 to 5.2 μg/mL of vitamin E with molar absorptivity of 1.23 × 104 L mol-1 cm-1 and the Sandell′s sensitivity was 0.0351 μg/cm2. The coefficient of determination was r2 = 0.997 with the relative standard deviation 1.1 %. The various factors which affect the resulting complex were optimized to obtain maximum absorption. The proposed method is satisfactorily utilized for pure as well as various dosage forms of vitamin E in pharmaceutical formulations which contains common reductants by pre-treatment of the samples.

Keywords

Spectrophotometer, Vitamin E, Ferric chloride, Dimethyl glyoxime

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