Amperometric Detection of Urea by Polyaniline and Poly(O-anisidine) Film under Galvanostatic Method: A Comparative Study

S.A. Folane1, K.S. Paithankar1,*, V.B. Deshmukh1, U.N. Shelke1, S.B. Iyyer1 and V.K. Gade2

1Department of Physics Research Center, Ahmednagar College, Ahmednagar-414001, India

2Department of Physics, Shri Anand College, Pathardi-414102 India

*Corresponding author: E-mail:


The growth mechanism of polyaniline (PANI) and poly(O-anisidine) (POA) with supporting electrolyte HClO4 on indium tin oxide (ITO) substrate has been investigated. The polymer film of PANI and POA deposited on indium tin oxide substrate have been synthesized by electrochemical polymerization method under galvanostatic condition in aqueous solution with supporting electrolyte HClO4 at 27 ºC. To compare the amperometric response of PANI and POA film on aurease enzyme in conventional sensor, the organized materials were characterized by analytical techniques such as UV-visible, FTIR and FE-SEM analyses. The performance of developed sensor was evaluated and the obtained urea biosensor exhibited shorter response time (3 s), wider range 1 × 10-9 to 9 ×10-9 M and the detection limit found to be 1 × 10-9 M. About 80 % of enzyme activity is retained for about 40 days. Modified sensor gives better result by polyaniline than poly(O-anisidine) sensor.


Polyaniline, Poly(O-anisidine), Galvanostatic.

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