Green Synthesis, Characterization and Antimicrobial Activity of Zinc Oxide Nanoparticles Using Root Extract of Viola canescens Wall. ex. Roxb.

Arun Kumar Khajuria1,*, Abha Negi2, NS Bisht1, V Maurya2 and Anuj Kandwal3

1Department of Botany, H.N.B. Garhwal University, Pauri Campus, Pauri-246001, India

2Department of Botany and Microbiology, H.N.B. Garhwal University, Chauras Campus, Srinagar-246174, India

3Department of Chemistry, H.N.B. Garhwal University, Pauri Campus, Pauri-246001, India

*Corresponding author: E-mail:


Stable zinc oxide nanoparticles were synthesized by using green approach of nanotechnology. The present investigation aimed to synthesize, stable, cost effective, rapid, eco-friendly approach for the bio-reduction of zinc nitrate hexahydarate to their nano size using phytochemicals present in the root extract of Viola canescens. The synthesized nanoparticles were further characterized by UV-visible, XRD, FTIR, SEM techniques and tested against the Gram-negative and Gram-positive pathogenic cultures of bacteria. The average size of synthesized nanoparticles was less than 11 nm with hexagonal morphology. The clear zone of inhibition against tested bacteria showed their capability as antimicrobial agent.


Zinc oxide nanoparticles, Root extract, XRD, FTIR SEM, Antimicrobial activity.

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