Isolation and Identification of Pure Bioactive Compounds from Cuscuta reflexa Grown on Nerium oleander Host Plant by Flash Column Chromatography and GC-MS/MS Analysis

Pooja Saini1,*, Ekta Menghani1 and Rekha Mithal2

1Department of Biotechnology, JECRC University, Jaipur-303905, India

2Department of Chemistry, JECRC University, Jaipur-303905, India

*Corresponding author: E-mail:


In this work, an ethyl acetate extract of Cuscuta reflexa grown on Nerium oleander host plant was used for the isolation and identification of pure bioactive compounds. Chloroform:methanol was used as mobile phase for flash column chromatography. Five pure bioactive compounds were isolated and two compounds were identified using GC-MS/MS technique. Glycerol-1-palmitate and squalene were identified in Cuscuta reflexa using NIST library on the basis of area percentage.


Cuscuta reflexa, Column chromatography, Bioactive compounds, GC-MS/MS Scanning, Glycerol-1-palmitate, Squalene.

   View Article PDF File Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.