Isolation and Identification of Pure Bioactive Compounds from Cuscuta reflexa Grown on Nerium oleander Host Plant by Flash Column Chromatography and GC-MS/MS Analysis

Pooja Saini1,*, Ekta Menghani1 and Rekha Mithal2

1Department of Biotechnology, JECRC University, Jaipur-303905, India

2Department of Chemistry, JECRC University, Jaipur-303905, India

*Corresponding author: E-mail: pooja.0024@gmail.com

Abstract

In this work, an ethyl acetate extract of Cuscuta reflexa grown on Nerium oleander host plant was used for the isolation and identification of pure bioactive compounds. Chloroform:methanol was used as mobile phase for flash column chromatography. Five pure bioactive compounds were isolated and two compounds were identified using GC-MS/MS technique. Glycerol-1-palmitate and squalene were identified in Cuscuta reflexa using NIST library on the basis of area percentage.

Keywords

Cuscuta reflexa, Column chromatography, Bioactive compounds, GC-MS/MS Scanning, Glycerol-1-palmitate, Squalene.

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