Isolation of Kaempferol from Vietnamese Ginkgo biloba Leaves by Preparative Column Chromatography

Tuong Van Nguyen1,* and Vinh Dinh2

1Faculty of Pharmacy, Nguyen Tat Thanh Univeristy, Ho Chi Minh City, Vietnam

2Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam

*Corresponding author: E-mail: ntvan@ntt.edu.vn

Abstract

The aim of this research was to develop a simple procedure to isolate kaempferol from Ginkgo biloba leaves extract. Flavonoids present in Ginkgo biloba leaves were extracted by 96 % ethanol. These flavonol glycosides which were hydrolyzed in the presence of HCl to convert to aglycones. The flavonol aglycones were extracted by the hydrolysis of solution with ethyl acetate which was evaporated to dryness under reduced pressure to obtain concentrated flavonol aglycones (C2). Flavonol aglycones were then purified by preparative column chromatography with a mobile phase composed of petroleum ether-ethyl acetate to acquire fraction C3. The recrystallization of fraction C3 in acetone realizes to have an amorphous sediment as fraction C4 that was supposed to be a mixture of kaempferol, quercetin and isorhamnetin. Kaempferol in this sediment was purified by column chromatography with a mobile phase composed of CHCl3-MeOH to achieve a fraction denoted as fraction C5. The isolated fraction C5 was assigned by its purity and structure by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and spectroscopic data analyses.

Keywords

Ginkgo leaves extract, Kaempferol, Silica gel, Column chromatography.

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