Sterols and Other Metabolites from Freshwater Microalga Chlorococcum infusionum (Schrank) Meneghini

Consolacion Y. Ragasa1,2,*, Emelina H. Mandia3,4, Maria Carmen S. Tan1, Glenn G. Oyong5, Cynthia F. Madrazo6, Aristotle T. Ubando7,8, Alvin B. Culaba7,8 and Chien-Chang Shen9

1Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines

2Department of Chemistry, De La Salle University Science & Technology Complex, Leandro V. Locsin Campus, Biñan City, Laguna 4024, Philippines

3Department of Biology, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines

4Microalgae Systematics and Applied Phycology Research Unit, Center for Natural Science and Environment Research (CENSER), De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines

5Molecular Science Unit, Center for Natural Science and Environmental Research, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines

6Department of Chemical Engineering, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines

7Department of Mechanical Engineering, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines

8Center for Engineering and Sustainable Development Research, College of Engineering, De La Salle University, Manila, Philippines

9National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1, Li-Nong St., Sec. 2, Taipei 112, Taiwan

*Corresponding author: Tel/Fax: +63 2 5360230; E-mail: consolacion.ragasa@dlsu.edu.ph

Abstract

Chemical investigation of the dichloromethane extract of Chlorococcum infusionum afforded a mixture of chondrillasterol (1) and 22-dihydrochondrillasterol (2) in a 3:1 ratio as the major sterols, and lutein (3), triacylglycerols (4), fatty acids (5) and hydrocarbons (6) as the minor compounds. The structures of compounds 1 and 2 were elucidated by extensive 1D and 2D NMR spectroscopy, while compounds 3-6 were identified by comparison of their NMR data.

Keywords

Chlorococcum infusionum, Phytosterols, Chondrillasterol, 22-Dihydrochondrillasterol, Lutein, Triacylglycerols.

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