Chemical Composition Analysis and Evaluation of Antibacterial Activities of Essential Oil from Clove (Syzygium aromaticum) Buds

Tran Quoc Toan1,2,*, Cam Thi Inh1, Hoang Thi Bich1, Pham Quoc Long1,2, Tran Thi Kim Ngan3,4 and Pham Thi Hai Ha5,*

1Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam

2Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam

3Center of Excellence for Biochemistry and Natural Products, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

4NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

5Department of Biotechnology, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

*Corresponding authors: E-mail: tranquoctoan2010@gmail.com; pthha@ntt.edu.vn

Abstract

Essential oils from Syzygium aromaticum (Myrtaceae) were extracted through hydrodistillation method, which applied in different fields such as relieve pain, promote healing, and aromatherapy industry. In this study, essential oil of clove buds was obtained using hydrodistillation method and evaluated for chemical compositions and physico-chemical characteristics. The averaged physico-chemical parameters were determined as specific gravity (1.0203 g/cm3), acid index (21.984), ester index (34.92). Moreover, the hydrodistillation process performance achieved by 6.85%. The composition of essential oils was analyzed by GC-MS, revealing a total of 15 volatile constituents existing in the Syzygium aromaticum oil. The major components were eugenol (76.542%), caryophyllene (4.319%), α-caryophyllene (0.456%), acete-ugenol (18.11%), caryophyllene oxide (0.192%), 4,4-dimethyl tetracyclo[6.3.2.0.(1,8)]tridecan-9-ol (0.12%). Syzygium aromaticum essential oil in this study was found to inhibit five bacteria strains including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa. The highest antimicrobial activity of clove essential oil was found for Bacillus cereus strain (14 mm).

Keywords

Clove buds, Syzygium aromaticum, Chemical composition, Antibacterial activities.

   View Article PDF File Under a Creative Commons License