Natural Phytochemicals and Biological Activities of Wild Grape (Ampelocissus martinii Planch.) Root Extract

P. Siripipatthana1,*,, P. Srihanam2, and A. Sangdee3

1Protein and Enzyme Technology Research Unit, Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand

2Creative and Innovation Chemistry Research Unit and Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand

3Microbiology and Applied Microbiology Research Unit, Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand

*Corresponding author: Tel./Fax: +66 43 754246; E-mail: patthraporn.s@msu.ac.th; nanasara1@gmail.com

Abstract

A hydromethanolic root extract of Ampelocissus martinii Planch. (A. martinii) was analyzed by standard methods for its phytochemical content, antioxidant activity, α-amylase and α-glucosidase inhibitions and antibacterial activities. The root extract exhibited the highest content of saponins, followed by phenols, proanthocyanidin and flavonoids, respectively. It showed high antioxidant activity in FRAP and CUPRAC assays. The root extract and standard Trolox had similar antioxidant activities in the DPPH and ABTS assay. It also showed much higher α-amylase and α-glucosidase inhibitory activity compared to standard acarbose. Moreover, the root extract inhibited all tested Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 6.25 mg/mL. These results indicate that A. martinii root can be pharmaceutically used as active ingredients to prevent bacterial infection and radical-related diseases especially diabetes.

Keywords

Phytochemicals, Enzyme inhibition, Antioxidant, Ampelocissus martinii.

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